Journal
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
Volume 55, Issue 4, Pages 1414-1419Publisher
AMER SOC MICROBIOLOGY
DOI: 10.1128/AAC.01835-09
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Funding
- Korean government (MEST) [2009-0071195]
- Korea Center for Disease Control [2009-E00520-00]
- National Research Foundation of Korea [2009-0071195] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
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Among 222 Proteus mirabilis clinical isolates collected from 17 hospitals in Korea in 2008, 28 (12.6%) and 8 (3.6%) isolates exhibited extended-spectrum beta-lactamase (ESBL) and AmpC phenotypes, respectively. The most common type of ESBL gene identified by PCR and sequencing experiments was bla(CTX-M-14a) (n = 12). The bla(CTX-M-90) (n = 4), bla(CTX-M-15) (n = 3), bla(CTX-M-12) (n = 3), bla(CTX-M-2) (n = 2), bla(CTX-M-14b) (n = 1), bla(TEM-52) (n = 5), and bla(SHV-12) (n = 1) genes were also detected. Eight isolates carried an AmpC beta-lactamase gene, such as bla(CMY-2) (n = 6) or bla(DHA-1) (n = 2). All bla genes encoding CTX-M-1- and CTX-M-9-type enzymes and all bla(CMY-2) genes were preceded by ISEcp1-like elements. The bla(CTX-M-2) gene found in two isolates was located on a complex class 1 integron. The bla(DHA-1) gene was preceded by a transcriptional regulator gene and was followed by phage shock protein genes. The bla(CTX-M) genes were located on the chromosome in 21 isolates. A plasmid location for the bla(CTX-M) gene was found in only four isolates: the bla(CTX-M-14a) gene was located on similar to 150-kbp IncA/C plasmids in three isolates and on a similar to 50-kbp IncN plasmid in one isolate. The bla(TEM-52) gene was located on similar to 50-kbp IncN plasmids in all five isolates. The AmpC beta-lactamase genes were located on the chromosome in seven of eight isolates; one isolate carried the bla(CMY-2) gene on a similar to 150-kbp IncA/C plasmid. Our results show that a chromosomal location of CTX-M ESBL and AmpC beta-lactamase genes in P. mirabilis is no longer an unusual phenomenon in hospital environments.
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