Journal
WORLD JOURNAL OF GASTROENTEROLOGY
Volume 11, Issue 40, Pages 6330-6337Publisher
BAISHIDENG PUBLISHING GROUP INC
DOI: 10.3748/wjg.v11.i40.6330
Keywords
Lactobacillus peptidoglycan; Gene expression; Immune response
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Funding
- PhD Programs Foundation of Ministry of Education of China [20040295005]
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AIM: To analyze the distinct immune responses induced by Lactobacillus peptidoglycan (PG). METHODS: BALB/c mice were intraperitoneally injected with PG once a day for three consecutive days. Peritoneal macrophage and splenocyte mRNA was extracted and the gene expression profile was studied using high-density oligonucleotide microarrays. Inhibitory effects of Lactobacillus PG on colon tumor tissue were studied in vitro and in vivo. RESULTS: The gene expression profiles revealed that the TLR-NF-kappa B and Jak-STAT signaling pathways were highly activated. An inflammatory phenotype was induced when peritoneal macrophages were initially exposed to Lactobacillus PG and switched to a more complex phenotype when BALB/c mice were treated with three doses of Lactobacillus PG. A protective physiological inflammatory response was induced after three consecutive days of PG treatment. It was tending toward Th1 dominant immune response. Lactobacillus PG also appeared to induce a significant in vivo anti-colon tumor effect. CONCLUSION: Lactobacillus PG is responsible for certain immune responses induced by Lactobacilli. Anti-tumor effects of Lactobacilli are likely to attribute to the activation of macrophages by PG expressed on the bacterial cell surface. (C) 2005 The WJG Press and Elsevier Inc. All rights reserved.
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