Journal
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
Volume 54, Issue 6, Pages 2666-2669Publisher
AMER SOC MICROBIOLOGY
DOI: 10.1128/AAC.01743-09
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- National Institute of Allergy and Infectious Diseases, National Institutes of Health, Department of Health and Human Services [N01-A1-30054, N01-A1-30055]
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Bovine-origin Escherichia coli isolates were tested for resistance phenotypes using a disk diffusion assay and for resistance genotypes using a DNA microarray. An isolate with gentamicin and amikacin resistance but with no corresponding genes detected yielded a 1,056-bp DNA sequence with the closest homologues for its inferred protein sequence among a family of 16S rRNA methyltransferase enzymes. These enzymes confer high-level aminoglycoside resistance and have only recently been described in Gram-negative bacteria.
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