Journal
GENETICA
Volume 125, Issue 2-3, Pages 205-210Publisher
SPRINGER
DOI: 10.1007/s10709-005-8419-0
Keywords
chromosome structure; fluorescent in situ hybridization (FISH); Pleuronectiformes; satellite DNA
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Agarose gels stained with Ethidium bromide and Southern blot experiments of HindIII-digested genomic DNA of Achirus lineatus evidenced the presence of monomers and multimers of a DNA segment of about 200 bp, named here Al-HindIII sequence. No signals were observed in Southern blot experiments with genomic DNA of other flatfish species. The DNA sequencing of four recombinant clones showed that Al-HindIII sequences had 204 bp and were 63.72% AT-rich. FISH experiments using a Al-HindIII sequence as probe showed bright signals in the centromeric position of all chromosomes of A. lineatus.
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