Journal
JOURNAL OF DENTAL RESEARCH
Volume 84, Issue 11, Pages 1070-1074Publisher
SAGE PUBLICATIONS INC
DOI: 10.1177/154405910508401120
Keywords
biomimetic coating; apatite; amelogenin; osteoblast; titanium implants
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Funding
- NIDCR NIH HHS [P60 DE 13076, DE 15332, DE 13414] Funding Source: Medline
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Osteoblast differentiation and extracellular matrix production are pivotal processes for implant osseointegration or bone tissue engineering. We hypothesized that a biomimetic coating on titanium surfaces, consisting of apatite and amelogenin, would promote such processes. Human Embryonic Palatal Mesenchymal pre-osteoblasts were used as a model for the evaluation of cell adhesion and spreading patterns, as well as mRNA expression of certain osteoblastic gene products. Real-time PCR showed significant (p < 0.05) increase in expression of type I collagen, alkaline phosphatase, an osteocalcin from cells grown on titanium with an apatite/amelogenin composite, as compared with that from cells grown on a pure titanium or apatite coating only. Osteocalcin expression was specifically stimulated by amelogenin added to the culture media. Enhanced attachment and cell spreading were also observed. The biomimetic coating promoting cell adhesion and osteoblast differentiation may have great potential for future dental and biomedical applications.
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