Journal
AMERICAN JOURNAL OF OBSTETRICS AND GYNECOLOGY
Volume 193, Issue 5, Pages 1716-1723Publisher
MOSBY, INC
DOI: 10.1016/j.ajog.2005.04.012
Keywords
placenta; trophoblast; insulin; adipophilin; fatty acids
Categories
Funding
- NICHD NIH HHS [HD-45671, HD-29190] Funding Source: Medline
- NIEHS NIH HHS [ES-11597] Funding Source: Medline
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Objective: This study was undertaken to test the hypothesis that insulin and fatty acids regulate adipophilin expression in Cultured human trophoblasts. Study design: Cytotrophoblasts isolated from term human placentas were Cultured in the absence or presence of insulin (10 nmol/L), and a mix of oleic and linoleic acid in serum-free medium. The expression of adipophilin as well as the fatty acid transport proteins (FATP) 2, 3, 4 and 6 was examined. Fat accumulation was quantified by BODIPY staining and fat Uptake determined Using [H-3]-oleic acid. Results: A combination of insulin and fatty acids enhanced the expression of adipophilin (2.3-fold, P < .05). In contrast, the expression of FATPs was unchanged. Furthermore, insulin and fatty acids increased the accumulation of fat droplets in trophoblasts by 4- to 5-fold (P < .05), but had no effect on oleic acid uptake. Conclusion: Insulin and fatty acids enhance the expression of adipophilin and the formation of fatty acid droplets in term human trophoblasts. (C) 2005 Mosby, Inc. All rights reserved.
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