Journal
PROTEIN AND PEPTIDE LETTERS
Volume 12, Issue 8, Pages 783-787Publisher
BENTHAM SCIENCE PUBL LTD
DOI: 10.2174/0929866054864175
Keywords
G protein; intein; expressed protein ligation; rhodopsin; transducin
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Funding
- NIGMS NIH HHS [GM08785] Funding Source: Medline
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Interactions between G proteins and GPCRs are fundamental for transmitting signals for a multitude of physiologic responses. Little is known regarding the protein-protein interface between the G protein and the receptor, much less the mechanisms for receptor activation of G proteins. Here, we will describe how expressed protein ligation will aid in the study of protein-protein interactions between semi-synthetic G alpha subunits and GPCRs.
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