Journal
FASEB JOURNAL
Volume 19, Issue 13, Pages 1798-1808Publisher
FEDERATION AMER SOC EXP BIOL
DOI: 10.1096/fj.05-4134com
Keywords
LTBP; TGF-beta; fibronectin null cells
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Funding
- NCI NIH HHS [CA34282, CA78422] Funding Source: Medline
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Transforming growth factor-beta s (TGF-beta) are secreted as latent complexes consisting of the TGF-beta dimer, the TGF-beta propeptide dimer, and the latent TGF-beta binding protein ( LTBP). Although the bonds between TGF-beta and its propeptide are cleaved intracellulary, the propeptide associates with TGF-beta by electrostatic interactions, thereby conferring latency to the complex. We reported that a specific sequence of LTBP-1 is required for latent TGF-beta activation by the integrin alpha v beta 6. Here we describe a 24 amino acid sequence from the hinge domain required for activation. The LTBP-1 polypeptide rL1N, which includes the hinge, associates with fibronectin in binding assays. We present evidence that fibronectin null cells minimally activate latent TGF-beta and poorly incorporate the active hinge sequence into their matrix. In addition, cells missing the fibronectin receptor alpha 5 beta 1 exhibit defective activation of latent TGF-beta by alpha v beta 6 and decreased matrix incorporation. The results indicate specificity for integrin-mediated latent TGF-beta activation that include unique sequences in LTBP-1 and an appropriate matrix molecule.
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