Journal
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
Volume 71, Issue 11, Pages 7556-7558Publisher
AMER SOC MICROBIOLOGY
DOI: 10.1128/AEM.71.11.7556-7558.2005
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PCR was used to rapidly identify and isolate 1-aminocyclopropane-1-carboxylate (ACC) deaminase genes from bacteria. The Shimodaira-Hasegawa test was used to assess whether phylogenetically anomalous gene placements suggestive of horizontal gene transfer (HGT) were significantly favored over vertical transmission. The best maximum likelihood (ML) ACC deaminase tree was significantly more likely than four alternative ML trees, suggesting HGT.
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