Journal
MARINE POLLUTION BULLETIN
Volume 50, Issue 11, Pages 1251-1261Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.marpolbul.2005.04.034
Keywords
biosensor; fecal indicators; fecal pollution; monitoring; RNA; water quality
Funding
- NIEHS NIH HHS [P30 ES005705, P50 ES012736, ES 05705, F32 ES005705, P50 ES012736-02] Funding Source: Medline
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Monitoring waters for indicator bacteria is required to protect the public from exposure to fecal Pollution. Our proof-of-concept study describes it method for detecting fecal coliforms. The coliform Escherichia coli was used as a model fecal indicator. DNA probe-coated magnetic beads in combination with the electrochemical monitoring of the oxidation state of guanine nucleotides should allow for direct detection of bacterial RNA. To demonstrate this concept, we used voltammetry in connection with pencil electrodes to detect isolated E. coli 16S rRNA. Using this approach, 10(7) cells of E. coli were detected in a quantitative, reproducible fashion in 4 h. Detection was achieved without a nucleic acid amplification step. The specificity of the assay for coliforms was demonstrated by testing against a panel of bacterial RNA. We also show that E. coli RNA can be detected directly from cell extracts. The method could be used for on-site detection and shows promise for adaptation into automated biosensors for water-quality monitoring. (c) 2005 Elsevier Ltd. All rights reserved.
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