Journal
INTERNATIONAL DAIRY JOURNAL
Volume 15, Issue 11, Pages 1122-1129Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.idairyj.2004.12.003
Keywords
PCR; cow; water buffalo; mozzarella cheese; 12S rRNA gene
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Polymerase chain reaction (PCR) has been applied for the specific detection of cows' DNA in water buffalo milk and mozzarella cheese by using primers targeting the mitochondrial 12S ribosomal RNA gene. The use of specific primers for cow yielded a 346 bp fragment from cows' milk DNA, whereas no amplification signal was obtained in sheep's, goats' and water buffalo's milk DNA. Analysis of both buffalo milk and buffalo mozzarella cheese mixtures containing different percentages of cows' milk or bovine mozzarella cheese, enabled the specific detection of cow's DNA with a sensitivity threshold of 0.1%. The proposed PCR assay represents a rapid and straightforward method for the detection of adulterations in water buffalo milk and mozzarella cheese. (c) 2005 Elsevier Ltd. All rights reserved.
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