Journal
AMERICAN JOURNAL OF HYPERTENSION
Volume 18, Issue 11, Pages 1503-1507Publisher
OXFORD UNIV PRESS
DOI: 10.1016/j.amjhyper.2005.05.033
Keywords
transient receptor potential channel; spontaneously hypertensive rats; calcium; siRNA
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Background: Disturbances in the regulation of cytosolic calcium concentration have been attributed to primary hypertension, but the role of calcium-permeable transient receptor potential canonical channel 3 (TRPC3) has not yet been evaluated in primary hypertension. Methods: Expression of TRPC3 was determined using in-cell Western assay. Evaluation of RNA interference for the downregulation of a specific gene in cells by small interfering RNA was performed. Measurements of cytosolic calcium were carried out using the fluorescent dye fura2. Results: Expression of TRPC3 was significantly increased in monocytes from spontaneously hypertensive rats (SHR) compared with normotensive Wistar-Kyoto rats (WKY). Transplasmamembrane calcium influx and thapsigargin-induced sustained calcium increase were significantly higher in SHR compared with WKY. In the presence of the TRP channel blocker SKF-96365 these differences were no longer observed. Specific TRPC3-knockdown by transfection of monocytes from SHR with small interfering RNA significantly reduced TRPC3 expression, trans-plasma membrane calcium influx, and thapsigargin-induced sustained calcium increase. Conclusions: This study shows, for the first time, increased TRPC3 channel expression and increased TRPC3-related calcium influx in SHR. Am J Hypertens 2005;18:1503-1507 (c) 2005 American Journal of Hypertension, Ltd.
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