Journal
JOURNAL OF NEUROSCIENCE RESEARCH
Volume 82, Issue 3, Pages 397-403Publisher
WILEY
DOI: 10.1002/jnr.20626
Keywords
lentiviral vectors; oligodendrocyte; morphology; myelin; transduction
Categories
Funding
- NIDCD NIH HHS [R01 DC002961, DC02961] Funding Source: Medline
- NINDS NIH HHS [P01 NS032636, R01 NS043205, R01 NS36265] Funding Source: Medline
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Lentiviral vectors are used widely to direct efficient gene transfer in vivo. We examined cell-specific expression in adult murine white matter after stereotaxic microinjection of four lentiviral constructs. We synthesized vesicular stomatitis virus glycoprotein (VSV-G) pseudotyped lentiviruses with combinations of two promoters, cytomegalovirus (CMV) or myelin basic protein (MBP), and two reporter sequences, cytosolic enhanced green fluorescent protein (eGFP) or a plasma membrane-targeted eGFP (human lymphocyte-specific protein tyrosine kinase [Lck]-eGFP). For all constructs, intracerebral injections to lateral corpus callosum resulted in widespread GFP expression in forebrain white matter glial cells. Intense cellular GFP fluorescence was observed within 3 days after injection and lasted for at least 28 days. The CMV promoter directed GFP expression in multiple glial cell types, whereas the MBP promoter targeted GFP specifically to oligodendrocytes. Expression of the membrane-targeted Lck-eGFP construct distinctly labeled individual myelinating processes of oligodendrocytes. Lentiviral constructs expressing eGFP or Lck-eGFP under the MBP promoter provide excellent visualization of oligodendrocyte morphology in intact white matter, and may prove valuable for delivering additional genes of interest to oligodendrocytes in vivo. (c) 2005 Wiley-Liss, Inc.
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