Journal
MOLECULAR AND CELLULAR BIOLOGY
Volume 25, Issue 21, Pages 9318-9323Publisher
AMER SOC MICROBIOLOGY
DOI: 10.1128/MCB.25.21.9318-9323.2005
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The heterotetrameric adaptor protein (AP) complexes AP-1, AP-2, AP-3, and AP-4 play key roles in transport vesicle formation and cargo sorting in post-Golgi trafficking pathways. Studies on cultured mammalian cells have shown that AP-2 mediates rapid endocytosis of a subset of plasma membrane receptors. To determine whether this function is essential in the context of a whole mammalian organism, we carried out targeted disruption of the gene encoding the mu 2 subunit of AP-2 in the mouse. We found that mu 2 heterozygous mutant mice were viable and had an apparently normal phenotype. In contrast, no mu 2 homozygous mutant embryos were identified among blastocysts from intercrossed heterozygotes, indicating that mu 2-deficient embryos die before day 3.5 postcoitus (E3.5). These results indicate that AP-2 is indispensable for early embryonic development, which might be due to its requirement for cell viability.
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