Journal
PLASMID
Volume 54, Issue 3, Pages 241-248Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.plasmid.2005.05.001
Keywords
promoters lepA, gsiB, xylA, and spac; htpG
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A series of plasmid-based expression vectors have been constructed allowing stable intracellular expression of recombinant proteins in Bacillus subtilis strains. These expression vectors are based on the recently described Escherichia coli-B. subtilis shuttle vector pMTLBS72 which replicates as theta circles. Besides the weak constitutive promoter P-lepA, we inserted three different controllable promoters: P-gsiB, which can be induced by heat and acid shock, and by ethanol, P-xylA and P-spac which respond to the addition of xylose and IPTG, respectively. The versatility of these expression vectors was demonstrated by fusing their promoters to a reporter gene and by overexpression of the HtpG protein with three of them. All recombinant vectors exhibited full structural stability. (c) 2005 Elsevier Inc. All rights reserved.
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