Journal
JOURNAL OF IMMUNOLOGY
Volume 175, Issue 9, Pages 5581-5585Publisher
AMER ASSOC IMMUNOLOGISTS
DOI: 10.4049/jimmunol.175.9.5581
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- NIAID NIH HHS [R01 AI47919] Funding Source: Medline
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The functional implication of molecular segregation within the immunological synapse remains uncertain. We recently reported that effector but not naive TCR transgenic murine CD8(+) T cells formed immunological synapses containing a central supramolecular activation cluster (cSAMC), suggesting that execution of effector functions such as cytolytic activity might be facilitated by the cSMAC structure. We have now explored this hypothesis using two approaches. First, by simultaneously imaging cSAMC formation and mobilization of cytotoxic granules to the synapse, we observed no correlation between the presence of a cSAMC and granule reorientation. Second, we took advantage of the observation that CD28 costimulation markedly enhances cSAMC formation. Granule polarization to the contact site was indistinguishable with B7-1(+) and B7-1(-) target cells, and cytolytic activity against B7-1(+) or B7-1(-) targets was similar and granule-dependent. Together, our results indicate that the formation of a cSAMC is not required for cytolytic activity in CD8(+) effector T cells.
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