Journal
NATURE METHODS
Volume 2, Issue 11, Pages 825-827Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/NMETH801
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Funding
- NEI NIH HHS [EY13574] Funding Source: Medline
- NHLBI NIH HHS [HL59198, HL73856] Funding Source: Medline
- NIBIB NIH HHS [EB00415] Funding Source: Medline
- NIDDK NIH HHS [DK35124, DK72517] Funding Source: Medline
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We synthesized a water-soluble, long-wavelength K+ sensor, TAC-Red, consisting of triazacryptand coupled to 3,6-bis(dimethylamino)xanthylium, whose fluorescence increased 14-fold at 0-50 mM K+ with K+-to-Na+ selectivity > 30. We visualized K+ waves in TAC-Red-stained brain cortex in mice during spreading depression, with velocity 4.4 +/- 0.5 mm/min, and K+ release and reuptake half-times (t(1/2)) of 12 +/- 2 and 32 +/- 4 s, respectively. Aquaporin-4 (AQP4) deletion slowed K+ reuptake about twofold, suggesting AQP4-dependent K+ uptake by astroglia.
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