Journal
MAGNETIC RESONANCE IN CHEMISTRY
Volume 43, Issue -, Pages S34-S39Publisher
WILEY
DOI: 10.1002/mrc.1688
Keywords
EPR spectroscopy; site-directed spin labelling; chemical synthesis of proteins; protein ligation
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The elucidation of structure and function of proteins and membrane proteins by EPR spectroscopy has become increasingly important in recent years as technological advances have been made in the design of spectrometers and in the chemistry of the nitroxide group. These new developments have increased the demand for tailor-made amino acids carrying a spin label on the one hand and for reliable methods for their incorporation into proteins on the other. Here we describe methods for site-specific spin labelling of proteins. It is shown that a combination of recombinant synthesis of proteins with chemically produced peptides (expressed protein ligation) allows the preparation of site-specifically spin-labelled proteins. Copyright (c) 2005 John Wiley & Sons, Ltd.
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