Journal
ANTI-CANCER DRUGS
Volume 20, Issue 4, Pages 238-248Publisher
LIPPINCOTT WILLIAMS & WILKINS
DOI: 10.1097/CAD.0b013e328326472e
Keywords
CK2; doxorubicin; emodin; inhibitor; protein kinases; resorufin
Categories
Funding
- Danish Cancer Society [DP06083, DP07109]
- NOVO NORDISK FONDEN
- Danish Medical Research Council [271-07-0464]
- Danish Research School in Molecular Cancer Research
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Screening a natural compound library led to the identification of resorufin as a highly selective and potent inhibitor of protein kinase CK2. Out of 52 kinases tested, only CK2 was inhibited, in contrast to emodin, a structurally related, known CK2 inhibitor that, in addition to CK2, inhibited ten other kinases by 90%. The IC50 values determined for the CK2 holoenzymes were 1.5 mu mol/l and for the free catalytic subunits ca. 4 mu mol/l. Altogether four cell lines were subjected to resorufin and emodin treatment. In the case of the three prostate carcinoma cell lines (PC-3, DU-145, LNCaP), 24 h treatment with 40 mu mol/l resorufin led to 15-20% dead cells; however, no caspase-mediated apoptosis was observed. In the case of the colorectal carcinoma HCT116 cell line, a similar picture was obtained, yet when resorufin was administered to cells treated with doxorubicin, apoptosis was strongly induced within 24 h. Endogenous protein kinase CK2 was inhibited by resorufin by ca. 80% in the three prostate cell lines. In the case of the HCT116 cells, the inhibition was only 40% supporting the notion of cell line-specific selectivity. Moreover, we analysed the effect of resorufin and emodin on selected signalling molecules in the cell lines under investigation. Anti-Cancer Drugs 20:238-248 (C)) 2009 Wolters Kluwer Health | Lippincott Williams & Wilkins.
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