Journal
PHOTOSYNTHESIS RESEARCH
Volume 86, Issue 1-2, Pages 123-130Publisher
SPRINGER
DOI: 10.1007/s11120-005-5301-y
Keywords
bacteriochlorophyll; Chlorobium vibrioforme; chlorosome; green sulfur photosynthetic bacteria; homolog; sodium sulfide
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Green sulfur photosynthetic bacteria Chlorobium (Chl.) vibrioforme (DSM 263 strain and NCIB 8327 substrain possessing BChl-c) and Chl. tepidum ( ATCC 49652) were photoautotrophically grown in liquid cultures containing different concentrations of sodium sulfide (Na2S). BChl-c homologs possessing a methyl group at the 12-position tended to increase in cells of the two strains of Chl. vibrioforme cultured under high Na2S concentrations. In contrast, the Na2S concentration in liquid cultures did not affect the relative composition of BChl-c homologs in Chl. tepidum. 8-Propyl-12- methyl([ P, M])-BChl-c homolog, which has been little observed in usual cultivations, could be isolated by reverse-phase high-performance liquid chromatography from the cells of Chl. vibrioforme grown under high Na2S contents. The [P, M]-BChl-c homolog has the R-configuration at the 3(1)-position, which was determined by H-1-NMR analyses.
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