Journal
JOURNAL OF PROTEOME RESEARCH
Volume 4, Issue 6, Pages 2355-2359Publisher
AMER CHEMICAL SOC
DOI: 10.1021/pr050245g
Keywords
glyco-affinity precipitation; protein purification; glycoconjugate; elastin-like polypeptide
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A one-pot affinity precipitation purification of carbohydrate-binding protein was demonstrated by designing thermally responsive glyco-polypeptide polymers, which were synthesized by selective coupling of pendant carbohydrate groups to a recombinant elastin-like triblock protein copolymer (ELP). The thermally driven inverse transition temperature of the ELP-based triblock polymer is maintained upon incorporation of carbohydrate ligands, which was confirmed by differential scanning calorimetry and H-1 NMR spectroscopy experiments. As a test system, lactose derivatized ELP was used to selectively purify a galactose-specific binding lectin through simple temperature-triggered precipitation in a high level of efficiency. Potential opportunities might be provided for enhanced proteomic, cell isolation as well as pathogen detection applications.
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