Journal
INTERNATIONAL JOURNAL OF UROLOGY
Volume 22, Issue 9, Pages 867-877Publisher
WILEY
DOI: 10.1111/iju.12829
Keywords
centromere proteinF; microRNA; microRNA-205; prostate cancer; tumor suppressor
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Funding
- KAKENHI [24592590, 25293333]
- Futaba Electronics Memorial Foundation
- Grants-in-Aid for Scientific Research [26462430, 25293333] Funding Source: KAKEN
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ObjectivesTo investigate the functional roles of microRNA-205 in the modulation of novel cancer pathways in prostate cancer cells. MethodsFunctional studies of microRNA-205 were carried out to investigate cell proliferation, migration and invasion in prostate cancer cell lines (PC3 and DU145) by restoration of mature microRNA. Insilico database and genome-wide gene expression analyses were carried out to identify molecular targets and pathways mediated by microRNA-205. Loss-of-function studies were applied to microRNA-205 target genes. ResultsRestoration of microRNA-205 in cancer cell lines significantly inhibited cancer cell migration and invasion. Our data showed that the centromere protein F gene was overexpressed in prostate cancer clinical specimens and was a direct target of microRNA-205 regulation. Silencing of centromere protein F significantly inhibited cancer cell migration and invasion. Furthermore, MCM7, an oncogenic gene functioning downstream of centromere protein F, was identified by si-centromere protein F transfectants in prostate cancer cells. ConclusionsLoss of tumor-suppressive microRNA-205 seems to enhance cancer cell migration and invasion in prostate cancer through direct regulation of centromere protein F. Our data describing pathways regulated by tumor-suppressive microRNA-205 provide new insights into the potential mechanisms of prostate cancer oncogenesis and metastasis.
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