Journal
PLANT CELL REPORTS
Volume 24, Issue 9, Pages 549-555Publisher
SPRINGER
DOI: 10.1007/s00299-005-0967-3
Keywords
Brassica napus; hypocotyls; transgenic plants; chitinase gene; scorpion toxin gene; insect resistance; diamondback moth (Plutella maculipenis) larvae
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Transgenic plants with introduced pest-resistant gene offer an efficient alternative insect control. The novel insect-resistant gene combination, chitinase(chi) and BmkIT(Bmk), containing an insect-specific chitinase gene and a scorpion insect toxin gene was introduced into Brassica napus cultivar via Agrobacterium-mediated transformation. Fifty-seven regenerated plantlets with kanamycin-resistance were obtained. Transgenic plants were verified by Southern blot analysis. Enzyme-linked immunosorbent assay (ELISA) and bioassay of artificial inoculation with diamondback moth (Plutella maculipenis) (DBM) larvae indicated that some of the transgenic plants were high-level expression for both chitinase and scorpion toxin proteins and performed high resistance against the tested pest infestation. The genetic analysis of T-1 progeny confirmed that the inheritance of introduced genes followed the Mendelian rules.
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