Journal
ENZYME AND MICROBIAL TECHNOLOGY
Volume 37, Issue 6, Pages 663-668Publisher
ELSEVIER SCIENCE INC
DOI: 10.1016/j.enzmictec.2005.07.026
Keywords
chitinases; lysozyme; enzymatic synthesis; chitooligosaccharides; organic solvents
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Diacetyl-chitobiose (GlcNAc)(2) was produced exclusively using a Vibrio furnissii chitinase (chi E, 89 kDa) hydrolysis of chitin treated with dimethylsulfoxide containing lithium chloride. This method represents a novel way for the preparation of colloidal chitin. Continuous diafiltration was used for the efficient recycling of enzyme and synthesis of (GlcNAc)(2) on a preparative scale. Chitooligosaccharides were also synthesized in a transglycosylation reaction employing lysozyme from hen-egg white with diacetyl-chitobiose produced by a Vibrio furnissii chitinase (chi E, 89 kDa) as a substrate in 50% 2-ethoxy ethyl ether co-solvent system. The co-solvent systems studied were triethyl phosphate, 2-ethoxy ethyl ether and acetone. (c) 2005 Elsevier Inc. All rights reserved.
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