Effect of seed pretreatment by magnetic field on the sensitivity of cucumber (Cucumis sativus) seedlings to ultraviolet-B radiation

The effects of seed pretreatment by magnetic field (MF) on the impacts of ultraviolet-B (UV-B) radiation were tested using cucumber (Cucumis sativus) seedlings in phytotron. Soaked cucumber seeds were placed in MF of various strengths (0, 0.2 and 0.45 T). After germination the seeds were sowed in homogeneous garden soil and grown, then cucumber seedlings were exposed to 0 (as control) and 3.5 kJ m(-2) UV-B irradiation, respectively. Some effects of UV-B radiation and MF-pretreatment as well as their combination were investigated. W-pretreatment increased seed germination rate, seedling growth and development, although also increased lipid oxidation and ascorbic acid contents. On the other hand, our results provided evidence that seed MF-pretreatment increased the sensitivity of cucumber seedlings to UV-B radiation. The seedling growth and development were significantly decreased by the combination of UV-B irradiation and MF-pretreatment. This combination also increased oxidative pressure and decreased actual quantum yield of PS II. Leaf UV-B absorbing compound was increased by MF-pretreatment or UV-B irradiation, whereas their combination significantly decreased it. These results suggested that the harmful effects of combination were partially due to the inhibition of secondary metabolism. (c) 2004 Elsevier B.V. All rights reserved.