Journal
JOURNAL OF CELL BIOLOGY
Volume 171, Issue 3, Pages 437-445Publisher
ROCKEFELLER UNIV PRESS
DOI: 10.1083/jcb.200506185
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Funding
- NCI NIH HHS [R01 CA096986, R01 CA095221, 1 R01 CA96986-01A1, 1 R01 CA095221-01A1] Funding Source: Medline
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In mammalian cells, the centrosome consists of a pair of centrioles and amorphous pericentriolar material. The pair of centrioles, which are the core components of the centrosome, duplicate once per cell cycle. Centrosomes play a pivotal role in orchestrating the formation of the bipolar spindle during mitosis. Recent studies have linked centrosomal activity on centrioles or centriole-associated structures to cytokinesis and cell cycle progression through G1 into the S phase. In this study, we have identified centrobin as a centriole-associated protein that asymmetrically localizes to the daughter centriole. The silencing of centrobin expression by small interfering RNA inhibited centriole duplication and resulted in centrosomes with one or no centriole, demonstrating that centrobin is required for centriole duplication. Furthermore, inhibition of centriole duplication by centrobin depletion led to impaired cytokinesis.
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