Journal
FEBS LETTERS
Volume 579, Issue 27, Pages 6074-6078Publisher
WILEY
DOI: 10.1016/j.febslet.2005.09.073
Keywords
chalcone isomerase; flavonoid biosynthesis; flower color; RNAi; Nicotiana tabacum
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A cDNA encoding chalcone isomerase (CHI) was isolated from the petals of Nicotiana tabacum and the effect of its suppression on flavonoid biosynthesis was analyzed in transgenic tobacco plants. CHI-suppression by RNA interference (RNAi) showed reduced pigmentation and change of flavonoid components in flower petals. The plants also accumulated high levels of chalcone in pollen, showing a yellow coloration. Our results first demonstrated that suppression of CHI by genetic transformation is possible in higher plants. This suggests that CHI plays a major part in the cyclization reaction from chalcone to flavanone, and that spontaneous reactions are few, if any, in tobacco plants. (c) 2005 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
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