Journal
PHYSICAL REVIEW LETTERS
Volume 95, Issue 20, Pages -Publisher
AMER PHYSICAL SOC
DOI: 10.1103/PhysRevLett.95.208101
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Funding
- NIGMS NIH HHS [GM38509] Funding Source: Medline
- Division Of Physics
- Direct For Mathematical & Physical Scien [0852130] Funding Source: National Science Foundation
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We report single-DNA-stretching experiments showing that the protein Fis, an abundant bacterial chromosome protein of E. coli, mediates a dramatic DNA condensation to zero length. This condensation occurs abruptly when DNA tension is reduced below a protein-concentration-dependent threshold f(*)< 1 pN. Following condensation, reopening under larger forces proceeds via a series of discrete jumps, indicating that Fis is able to stabilize DNA crossings. Our experiments suggest that Fis may play a role in vivo stabilizing the loop-domain structure of the bacterial chromosome.
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