Journal
TETRAHEDRON
Volume 61, Issue 46, Pages 10908-10916Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.tet.2005.08.105
Keywords
hydroxynitrile-lyase; Prunus mume; cyanohydrins; asymmetric synthesis
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A new hydroxynitrile lyase (HNL) was isolated from the seed of Japanese apricot (Prunus mume). The enzyme has similar properties with HNL isolated from other Prunus species and is FAD containing enzyme. It accepts a large number of unnatural substrates (benzaldehyde and its variant) for the addition of HCN to produce the corresponding cyanohydrins in excellent optical and chemical yields. A new HPLC based enantioselective assay technique was developed for the enzyme, which promotes the addition of KCN to benzaldehyde in a buffered solution (pH = 4.5). (c) 2005 Elsevier Ltd. All rights reserved.
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