Journal
ANALYTICAL CHEMISTRY
Volume 77, Issue 22, Pages 7274-7281Publisher
AMER CHEMICAL SOC
DOI: 10.1021/ac050891z
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A chemical derivatization method, amidination, that has recently been effectively employed in peptide mass spectrometry experiments is used to covalently modify lysines in several standard proteins. Protein and peptide mass spectra identify sites at which the reaction does or does not occur. Ibis is therefore a rapid approach to elucidate solvent-accessible regions of folded proteins.
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