Journal
ANNUAL REVIEW OF PHYSICAL CHEMISTRY, VOL 62
Volume 62, Issue -, Pages 507-530Publisher
ANNUAL REVIEWS
DOI: 10.1146/annurev.physchem.012809.103512
Keywords
label-free imaging; coherent Raman spectroscopy; stimulated Raman scattering microscopy; stimulated emission microscopy; pump-probe microscopy
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Funding
- NATIONAL INSTITUTE OF BIOMEDICAL IMAGING AND BIOENGINEERING [R01EB010244] Funding Source: NIH RePORTER
- NIBIB NIH HHS [1R01EB010244-01, R01 EB010244-02, R01 EB010244] Funding Source: Medline
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The quest for ultrahigh detection sensitivity with spectroscopic contrasts other than fluorescence has led to various novel approaches to optical microscopy of biological systems. Coherent nonlinear optical imaging, especially the recently developed nonlinear dissipation microscopy (including stimulated Raman scattering and two-photon absorption) and pump-probe microscopy (including excited-state absorption, stimulated emission, and ground-state depletion), provides new image contrasts for nonfluorescent species. Thanks to the high-frequency modulation transfer scheme, these imaging techniques exhibit superb detection sensitivity. By directly interrogating vibrational and/or electronic energy levels of molecules, they offer high molecular specificity. Here we review the underlying principles and excitation and detection schemes, as well as exemplary biomedical applications of this emerging class of molecular imaging techniques.
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