Journal
JOURNAL OF CHROMATOGRAPHY A
Volume 1095, Issue 1-2, Pages 60-67Publisher
ELSEVIER
DOI: 10.1016/j.chroma.2005.07.123
Keywords
sulfonamides; chemiluminescence; HPLC; bis[4-nitro-2-(3,6,9-trioxadecyloxycarbonyl)phenyl] oxalate; milk
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The determination of seven sulfonamides by means of HPLC with chemiluminescence detection is proposed for the first time. The analytes are derivatized with fluorescamine, separated and subsequently they participate in the post-column chemiluminescence (CL) peroxyoxalate system using imidazole as a catalyst. Among the different peroxyoxalates tested, bis[4-nitro-2-(3,6,9-trioxadecyloxycarbonyl)phenyl] oxalate provides higher sensitivities and stabilities, avoiding precipitation problems. A rigorous optimization of the significant variables by means of experimental designs has been developed in order to reconcile the chromatographic conditions with the CL reaction. The method provides detection limits in the low mu gl (-1) range and has been satisfactorily applied to the analysis of spiked raw milk samples. (c) 2005 Elsevier B.V. All rights reserved.
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