Journal
FEBS LETTERS
Volume 579, Issue 28, Pages 6423-6427Publisher
WILEY
DOI: 10.1016/j.febslet.2005.09.103
Keywords
protein synthesis; antibiotics; ribosome; mitochondria; E. coli
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All medically useful antibiotics should have the potential to distinguish between target microbes (bacteria) and host cells. Although many antibiotics that target bacterial protein synthesis show little effect on the translation machinery of the eukaryotic cytoplasm, it is unclear whether these antibiotics target or not the mitochondrial translation machinery. We employed an in vitro translation system from bovine mitochondria, which consists of mitochondrial ribosomes and mitochondrial elongation factors, to estimate the effect of antibiotics on mitichondrial protein synthesis. Tetracycline and thiostrepton showed similar inhibitory effects on both Escherichia coli and mitochondrial protein synthesis. The mitochondrial system was more resistant to tiamulin, macrolides, virginiamycin, fusidic acid and kirromycin than the E. coli system. The present results, taken together with atomic structure of the ribosome, may provide useful information for the rational design of new antibiotics having less adverse effects in humans and animals. (c) 2005 Published by Elsevier B.V. on behalf of the Federation of European Biochemical Societies.
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