Synthesis of a new hydrophilic o-nitrobenzyl photocleavable linker suitable for use in chemical proteomics

Linkers currently used in solid phase synthesis are generally short and hydrophobic, limiting their usefulness in biological systems. Herein, we describe a facile synthesis of a long, hydrophilic, o-nitrobenzyl photocleavable linker, suitable for constructing affinity supports for use in chemical proteomics. The rates of photolysis of the linker on exposure to UV light emitting diodes are reported. (c) 2005 Elsevier Ltd. All rights reserved.