Journal
WORLD JOURNAL OF GASTROENTEROLOGY
Volume 11, Issue 43, Pages 6867-6870Publisher
BAISHIDENG PUBL GRP CO LTD
DOI: 10.3748/wjg.v11.i43.6867
Keywords
MSX2; Caspase-3; Gemcitabine
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Funding
- Ministry of Education, Science, Sports and Culture in Japan [14370172, 15590615]
- Grants-in-Aid for Scientific Research [15590615, 14370172] Funding Source: KAKEN
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AIM: To evaluate the effect of MSX2 on gemcitabine-induced caspase-3 activation in pancreatic cancer cell line Panc-1. METHODS: Using V5-tagged MSX2 expression vector, stable transfectant of MSX2 was generated from Panc-1 cells (Px14 cells). Cell viability under gemcitabine administration was determined by MTT assay relative to control cell line (empty-vector transfected Panc-1 cells; P-3EV cells). Hoechst staining was used for the detection of apoptotic cell. Activation of caspase-3 was assessed using Western blotting analysis and direct measurement of caspase-3 specific activities. RESULTS: MSX2 overexpression in Panc-1 cells resulted in decreased gemcitabine-induced caspase-3 activation and increased cell viability under gemcitabine treatment in Px14 cells. CONCLUSION: MSX2 exerts repressive effects on gemcitabine-induced apoptotic pathway. This novel apoptosis-regulating function of MSX2 may provide a new therapeutic target for pancreatic cancer. (C) 2005 The WJG Press and Elsevier Inc. All rights reserved.
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