Journal
BIOCHEMICAL JOURNAL
Volume 392, Issue -, Pages 353-362Publisher
PORTLAND PRESS LTD
DOI: 10.1042/BJ20050890
Keywords
adenine nucleotide translocase (ANT); knock-out mouse; carboxyatractylate; Drosophila; proton leak
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Funding
- NINDS NIH HHS [R01 NS041850, NS41850] Funding Source: Medline
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The basal proton conductance of mitochondria causes mild uncoupling and may be an important contributor to metabolic rate. The molecular nature of the proton-conductance pathway is unknown. We show that the proton Conductance of muscle mitochondria from mice in which isoform I of the adenine nucleotide translocase has been ablated is half that of wild-type controls. Overexpression of the adenine nucleotide translocase encoded by the stress-sensitive B gene in Drosophila mitochondria increases proton conductance, and underexpression decreases it, even when the carrier is fully inhibited Using carboxyatractylate. We conclude that half to two-thirds of the basal proton conductance of mitochondria is catalysed by the adenine nucleotide carrier, independently of its ATP/ADP exchange or fatty-acid-dependent proton-leak functions.
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