Journal
JOURNAL OF MEDICINAL CHEMISTRY
Volume 48, Issue 24, Pages 7513-7516Publisher
AMER CHEMICAL SOC
DOI: 10.1021/jm050431y
Keywords
-
Categories
Funding
- NIDA NIH HHS [P01 DA12408] Funding Source: Medline
Ask authors/readers for more resources
Novel fluorescent ligands were synthesized to identify a high-affinity probe that would enable visualization of the dopamine transporter (DAT) in living cells. Fluorescent tags were extended from the N- or 2-position of 2 beta-carbomethoxy-3 beta-(3,4-dichlorophenyl)tropane, using an ethylamino linker. The resulting 2-substituted (5) and N-substituted (9) rhodamine-labeled ligands provided the highest DAT binding affinities expressed in COS-7 cells (K-i = 27 and 18 nM, respectively) in the series. Visualization of the DAT with 5 and 9 was demonstrated by confocal fluorescence laser scanning microscopy in stably transfected HEK293 cells.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available