Journal
PROTEOMICS
Volume 5, Issue 18, Pages 4678-4683Publisher
WILEY
DOI: 10.1002/pmic.200500002
Keywords
immobilized metal-affinity chromatography; phosphoproteomics; phosphorylation; two-dimensional gel electrophoresis
Ask authors/readers for more resources
Phosphoproteome studies are hampered by the lack of methods which allow a comprehensive Received: January 4, 2005 and fast analysis of intact phosphoproteins. Here we describe an immobilized metal-affinity Revised: April 16, 2005 chromatography (IMAC)-based technique for the enrichment of phosphorylated proteins, which Accepted: April 25, 2005 allows recovery of up to 90% of phosphoproteins. This technique is compatible with 2-DE and can be applied to cultured cells and tissues.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available