Journal
CURRENT OPINION IN COLLOID & INTERFACE SCIENCE
Volume 10, Issue 5-6, Pages 245-249Publisher
ELSEVIER SCIENCE LONDON
DOI: 10.1016/j.cocis.2005.09.018
Keywords
cryo-EM; tomography; time resolution; Langmuir films
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Cryo-electron microscopy has evolved in an established approach to study the structure of bio-colloids. Recent developments ill instrumentation and automation, often demanded by life sciences, made cryo-EM a general tool in colloid chemistry. Recently improved instrumentation for vitrification has resulted in reliable and reproducible preparation of specimen in water and other solvents. A dynamic approach, following processes in time is gaining importance and a time resolution up to 1 ms is expected to become general available. With improved instrumentation and automation 3D reconstructions by cryo-EM tomography are becoming routinely accessible. Tomography as Such or in conjunction with time resolved microscopy is expected to give new insights in (macro)molecular assembly/disassembly mechanisms and thus become an essential tool in nano-chemistry. (c) 2005 Published by Elsevier Ltd.
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