Arsenate uptake and translocation in seedlings of two genotypes of rice is affected by external phosphate concentrations

Two genotypes of rice (Oryza saliva L.), 94D-54 and 94D-64 were used to investigate the formation of iron plaque controlled by different phosphorus (P) concentrations and the effect of iron plaque on arsenate uptake in a hydroponic experiment. External P concentrations from 10 to 50 mu M caused a marked decrease in dithionite-citrate-bicarbonate (DCB)-Fe concentrations for both genotypes, but further increases from 50 to 300 mu M only resulted in small decrease. Arsenic (As) concentrations in DCB-extracts were determined by the amounts of iron plaque and the adsorption capacity of As by iron plaque, and both controlled by external P concentrations. At 10 mu M external P, genotype 94D-54 had higher Fe, As and P concentrations in DCB-extracts than genotype 94D-64, but the difference disappeared with increasing P concentrations. Increasing P concentrations decreased the percentages of As distributed in iron plaque from around 70 to 10%, and increased the percentages of As in roots and shoots gradually from around 20 to 60% for toots and from 5 to nearly 35% for shoots, respectively. Moreover, P concentration increased the molar ratio of shoot-to-root As, from 0.05 to nearly 0.2, indicating P concentration may promote As translocation from roots to shoots. (C) 2005 Elsevier B.V. All rights reserved.