Journal
ANNUAL REVIEW OF BIOPHYSICS, VOL 43
Volume 43, Issue -, Pages 303-329Publisher
ANNUAL REVIEWS
DOI: 10.1146/annurev-biophys-051013-022836
Keywords
PALM; RESOLFT; PAGFP; PAmCherry; EosFP
Categories
Funding
- EUNICE KENNEDY SHRIVER NATIONAL INSTITUTE OF CHILD HEALTH & HUMAN DEVELOPMENT [ZIAHD001609, ZIAHD008850] Funding Source: NIH RePORTER
- NATIONAL CANCER INSTITUTE [R01CA164468] Funding Source: NIH RePORTER
- NATIONAL INSTITUTE OF BIOMEDICAL IMAGING AND BIOENGINEERING [R01EB013571] Funding Source: NIH RePORTER
- NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES [R01GM073913] Funding Source: NIH RePORTER
- Intramural NIH HHS [Z99 HD999999] Funding Source: Medline
- NCI NIH HHS [CA164468, R01 CA164468] Funding Source: Medline
- NIBIB NIH HHS [R01 EB013571, EB013571] Funding Source: Medline
- NIGMS NIH HHS [GM073913, R01 GM073913] Funding Source: Medline
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Superresolution fluorescence microscopy permits the study of biological processes at scales small enough to visualize fine subcellular structures that are unresolvable by traditional diffraction-limited light microscopy. Many superresolution techniques, including those applicable to live cell imaging, utilize genetically encoded photocontrollable fluorescent proteins. The fluorescence of these proteins can be controlled by light of specific wavelengths. In this review, we discuss the biochemical and photophysical properties of photocontrollable fluorescent proteins that are relevant to their use in superresolution microscopy. We then describe the recently developed photoactivatable, photoswitchable, and reversibly photoswitchable fluorescent proteins, and we detail their particular usefulness in single-molecule localization-based and nonlinear ensemble-based superresolution techniques. Finally, we discuss recent applications of photocontrollable proteins in superresolution imaging, as well as how these applications help to clarify properties of intracellular structures and processes that are relevant to cell and developmental biology, neuroscience, cancer biology and biomedicine.
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