Journal
ANNUAL REVIEW OF BIOPHYSICS, VOL 39
Volume 39, Issue -, Pages 429-448Publisher
ANNUAL REVIEWS
DOI: 10.1146/annurev.biophys.093008.131327
Keywords
DNA replication; DNA polymerase; helicase; primase; single-molecule biophysics
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Funding
- NIGMS NIH HHS [R01-GM-077248] Funding Source: Medline
- NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES [R01GM077248] Funding Source: NIH RePORTER
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Replication of DNA is carried out by the replisome, a multiprotein complex responsible for the unwinding of parental DNA and the synthesis of DNA on each of the two DNA strands. The impressive speed and processivity with which the replisome duplicates DNA are a result of a set of tightly regulated interactions between the replication proteins. The transient nature of these protein interactions makes it challenging to study the dynamics of the replisome by ensemble-averaging techniques. This review describes single-molecule methods that allow the study of individual replication proteins and their functioning within the replisome. The ability to mechanically manipulate individual DNA molecules and record the dynamic behavior of the replisome while it duplicates DNA has led to an improved understanding of the molecular mechanisms underlying DNA replication.
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