Journal
ANNUAL REVIEW OF BIOPHYSICS
Volume 38, Issue -, Pages 53-74Publisher
ANNUAL REVIEWS
DOI: 10.1146/annurev.biophys.050708.133634
Keywords
membrane organization; chemical composition imaging; NanoSIMS; ToF-SIMS; dynamic SIMS
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Funding
- U.S. Department of Energy [DE-AC52-07NA27344]
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Imaging mass spectrometry combines the power of miss spectrometry to identify complex molecules based on mass with simple imaging. Recent advances in secondary ion mass spectrometry have improved sensitivity and spatial resolution, so that these methods have the potential to bridge between high-resolution structures obtained by X-ray crystallography and cyro-electron microscopy and ultrastructure visualized by conventional light microscopy. Following background information on the method and instrumentation, we address the key issue of sample preparation. Because mass spectrometry is performed ill high vacuum, it is essential to preserve the lateral organization of the sample while removing bulk water, and this has been major barrier for applications to biological systems. Recent applications of imaging mass spectrometry to cell biology, microbial communi ties, and biosynthetic pathways are summarized briefly, and studies of biological membrane organization are described in greater depth.
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