Protein analysis of dwarfed transgenic rice plants overexpressing GA2-oxidase gene

Using 2-D electrophoresis, we analyzed proteins from transgenic rice overexpressing gibberellin acid (GA) catabolic enzyme, GA2-oxidase. These results indicate eight specific proteins differentially expressed in the transformed rice stems of T-1 generation, but non in case of T-2 generation. Proteins isolated from different stages of leaves of T-1 generation showed no significant differences, except one-month-old leaf, where five differentially expressed proteins are visible.