Journal
FEMS MICROBIOLOGY LETTERS
Volume 253, Issue 1, Pages 155-161Publisher
OXFORD UNIV PRESS
DOI: 10.1016/j.femsle.2005.09.032
Keywords
Salmonella; LAMP; rapid; sensitive; detection
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Loop-mediated isothermal amplification (LAMP) assay detected Salmonella within 60 min. The 220 strains of 39 serotypes of Salmonella subsp. enterica and 7 strains of Salmonella enterica subsp. arizonae were amplified, but not 62 strains of 23 bacterial species other than Salmonella. The sensitivity of the LAMP assay was found to be >2.2 cfu/test tube using nine serotypes. The specificity was similar to that of a PCR assay, but the sensitivity of LAMP was greater. Both fluorescence and turbidity were able to detect the products in the LAMP assay. S. enteritidis in a liquid egg sample artificially inoculated with the organism was detected by the LAMP assay at 2.8 cfu/test tube, although negative by PCR assay. These results indicate that the LAMP assay is a rapid, specific and sensitive detection method for Salmonella. (c) 2005 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved.
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