Journal
ANNUAL REVIEW OF ANALYTICAL CHEMISTRY, VOL 5
Volume 5, Issue -, Pages 293-315Publisher
ANNUAL REVIEWS
DOI: 10.1146/annurev-anchem-062011-143122
Keywords
self-renewal; temporal cues; development; cell patterning; transport analysis
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Funding
- NATIONAL INSTITUTE OF BIOMEDICAL IMAGING AND BIOENGINEERING [R01EB007278] Funding Source: NIH RePORTER
- NIBIB NIH HHS [EB007278, R01 EB007278] Funding Source: Medline
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Although stem cell fate is traditionally manipulated by exogenously altering the cells' extracellular signaling environment, the endogenous autocrine and paracrine signals produced by the cells also contribute to their two essential processes: self-renewal and differentiation. Autocrine and/or paracrine signals are fundamental to both embryonic stem cell self-renewal and early embryonic development, but the nature and contributions of these signals are often difficult to fully define using conventional methods. Microfluidic techniques have been used to explore the effects of cell-secreted signals by controlling cell organization or by providing precise control over the spatial and temporal cellular microenvironment. Here we review how such techniques have begun to be adapted for use with embryonic stem cells, and we illustrate how many remaining questions in embryonic stem cell biology could be addressed using microfluidic technologies.
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