Journal
ANNUAL REVIEW OF ANALYTICAL CHEMISTRY
Volume 1, Issue -, Pages 191-227Publisher
ANNUAL REVIEWS
DOI: 10.1146/annurev.anchem.1.031207.113100
Keywords
capillary electrophoresis; laser-induced fluorescence; chemical cytometry; cellular analytes
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Funding
- NIH [EB004436, EB004597, F32GM078768]
- NATIONAL INSTITUTE OF BIOMEDICAL IMAGING AND BIOENGINEERING [R01EB004597, R01EB004436] Funding Source: NIH RePORTER
- NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES [F32GM078768] Funding Source: NIH RePORTER
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Chemical analysis of single cells requires methods for quickly and quantitatively, detecting a diverse array, of analytes from extremely small volumes (femtoliters to nanoliters) with very high sensitivity and selectivity Microelectrophoretic separations, using, both traditional capillary electrophoresis and emerging microfluidic methods, are well suited for handling the unique size of single cells and limited numbers of intracellular molecules. Numerous analytes, ranging from small molecules such as amino acids and neurotransmitters to large proteins and subcellular organelles, have been quantified in single cells using microelectrophoretic separation techniques. Microseparation techniques, coupled to varying detection schemes including absorbance and fluorescence detection, electrochemical detection, and mass spectrometry, have allowed researchers to examine a number of processes inside single cells. This review also touches on a promising direction in single cell cytometry: the development of microfluidics for integrated cellular manipulation, chemical processing, and separation Of cellular contents.
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