Effects of pomiferin premedication on the antioxidant status of rats with ischemia-reperfused kidney

The aim of this study was to analyze the relationship between the antioxidant effect and the dose of pomiferin during a prophylactic administration. The pathological model for an in vivo experiment was the unilateral ischemia-reperfusion of the laboratory rat kidney. The animals were randomly divided into five groups. Pomiferin was administered orally at doses of 5, 10 and 20 mg center dot kg(-1), in 2 ml of Avicel solution by a gastric sound once a day to three premedicated groups. A placebo - 2 ml of 0.5% Avicel solution - was given to the fourth group, and the fifth group was intact. The premedication lasted 15 days and subsequently ischemia of the left kidney was incited under a general anaesthesia for 60 min. The reperfusion lasted 10 min and it was concluded with blood collection from the left ventricle, and the reperfused kidney was recovered. Selected biochemical markers were assessed in blood: superoxide dismutase, glutathione peroxidase, total antioxidant capacity and malondialdehyde. Kidney tissue samples were used for histopathological examination. Biochemical and histopathological results confirmed the anticipated effects of pomiferin. Porniferin supported the defensive reactions of the system against free radicals and decreased lipid peroxidation in cell membranes. The relation between the effect and the dose of pomiferin was not linear, and some of the assessed differences between the groups were statistically highly significant. The best results of the biochemical examination were achieved after the administration of pomiferin at the dose of 20 mg center dot kg(-1). The histopathological results confirmed the dose of 5 mg center dot kg(-l) to be the most effective one.