Journal
OPTICAL AND QUANTUM ELECTRONICS
Volume 37, Issue 13-15, Pages 1439-1445Publisher
SPRINGER
DOI: 10.1007/s11082-005-4223-4
Keywords
dorsal skin fold chamber; multiphoton imaging; second harmonic generation
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The advantages offered by multiphoton microscopy enabled this technique to be applied for in vivo understanding of physiological processes. However, multiphoton intravital microscopy also requires associated technologies to be developed. In this work, we detailed the design of a dorsal skin fold chamber made of titanium alloy that allow high resolution multiphoton fluorescence and second harmonic generation (SHG) microscopy to be achieved. We demonstrate that our apparatus is capable of obtaining high resolution, images of blood vessels and collagen matrix in nude mice. Such an imaging chamber will allow physiological processes to be investigated at high resolution.
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