Journal
JOURNAL OF CLINICAL MICROBIOLOGY
Volume 43, Issue 12, Pages 6147-6149Publisher
AMER SOC MICROBIOLOGY
DOI: 10.1128/JCM.43.12.6147-6149.2005
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Community-associated methicillin-resistant Staphylococcus aureus harboring Panton-Valentine leukocidin (PVL) genes is an emerging pathogen. A novel real-time PCR assay for identification of MRSA isolates containing PVL was developed. The PVL assay was used in a triplex format allowing simultaneous amplification of mecA, nuc, and PVL genes in 614 clinical isolates. This assay facilitates the rapid identification of PVL-positive isolates of MRSA.
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